腦室微量給藥導(dǎo)管可固定在大鼠、小鼠的顱骨上埋入腦部適當(dāng)?shù)纳疃?/span>配合相應(yīng)的PE給藥導(dǎo)管、微量注射器或者使用實現(xiàn)對大鼠、小鼠顱內(nèi)的微量給藥。

單套管顱內(nèi)給藥

整個套管由基座、注射內(nèi)管、導(dǎo)管帽、鎖緊螺帽組成

單套管顱內(nèi)給藥植入腦室的示意圖

給藥套管的詳細構(gòu)造及主要尺寸

（長度單位mm）

訂購指南需要確定以下幾個尺寸

D1導(dǎo)管外徑的尺寸；

L1下探到腦室套管的長度（含顱骨和腦室）

L3注射內(nèi)管探出基座套管的長度；

L4導(dǎo)管芯（也就是堵頭）探出基座套管的長度；

單套管的型號各個組成配件的貨號和尺寸

雙套管顱內(nèi)給藥

適用于兩種不同或相同的藥物注射在兩個不同的位置；

雙套管的主要組成

雙套管顱內(nèi)給藥植入腦室的示意圖

雙套管的詳細構(gòu)造及主要尺寸

訂購指南需要確定以下幾個尺寸

D1導(dǎo)管外徑的尺寸；

L1下探到腦室套管的長度（含顱骨和腦室）

P 兩根導(dǎo)管的間距

L3注射內(nèi)管探出基座套管的長度；

L4導(dǎo)管芯（也就是堵頭）探出基座套管的長度；

雙套管的型號各個組成配件的貨號和尺寸

新生鼠適用型號

小鼠、大鼠適用型

大鼠適用型

顱內(nèi)微量注射實驗還需要用到以下相關(guān)的設(shè)備和工具

腦立體定位儀、套管夾持器、、微量進樣器、PE給藥導(dǎo)管、固定螺絲等

腦立體定位儀可根據(jù)需求有多種型號可供選擇大鼠適配型、小鼠適配型、單臂、雙臂、數(shù)顯式、電動式

套管夾持器

微量進樣器

PE給藥導(dǎo)管

牙科水泥

固定螺絲

小動物

參考文獻

[1] Wahis J, Baudon A, Althammer F, et al. Astrocytes mediate the effect of oxytocin in the central amygdala on neuronal activity and affective states in rodents[J]. Nature neuroscience, 2021, 24(4)529-541.

[2] Zhu Z, Ng D W H, Park H S, et al. 3D-printed multifunctional materials enabled by artificial-intelligence-assisted fabrication technologies[J]. Nature Reviews Materials, 2021, 6(1)27-47.

[3] Wang L, Gillis-Smith S, Peng Y, et al. The coding of valence and identity in the mammalian taste system[J]. Nature, 2018, 558(7708)127-131.

[4] Terburg D, Scheggia D, Del Rio R T, et al. The basolateral amygdala is essential for rapid escapea human and rodent study[J]. Cell, 2018, 175(3)723-735. e16.

[5] Peng Y, Gillis-Smith S, Jin H, et al. Sweet and bitter taste in the brain of awake behaving animals[J]. Nature, 2015, 527(7579)512-515.

[6] Tye K M, Mirzabekov J J, Warden M R, et al. Dopamine neurons modulate neural encoding and expression of depression-related behaviour[J]. Nature, 2013, 493(7433)537-541.

[7] Sotres-Bayon F, Sierra-Mercado D, Pardilla-Delgado E, et al. Gating of fear in prelimbic cortex by hippocampal and amygdala inputs[J]. Neuron, 2012, 76(4)804-812.

[8] Pascoli V, Turiault M, Lüscher C. Reversal of cocaine-evoked synaptic potentiation resets drug-induced adaptive behaviour[J]. Nature, 2012, 481(7379)71-75.

[9] Lemos J C, Wanat M J, Smith J S, et al. Severe stress switches CRF action in the nucleus accumbens from appetitive to aversive[J]. Nature, 2012, 490(7420)402-406.

[10] Goshen I, Brodsky M, Prakash R, et al. Dynamics of retrieval strategies for remote memories[J]. Cell, 2011, 147(3)678-689.

[11] Aponte Y, Atasoy D, Sternson S M. AGRP neurons are sufficient to orchestrate feeding behavior rapidly and without training[J]. Nature neuroscience, 2011, 14(3)351-355.

[12] Kravitz A V, Freeze B S, Parker P R L, et al. Regulation of parkinsonian motor behaviours by optogenetic control of basal ganglia circuitry[J]. Nature, 2010, 466(7306)622-626.

[13] Amat J, Baratta M V, Paul E, et al. Medial prefrontal cortex determines how stressor controllability affects behavior and dorsal raphe nucleus[J]. Nature neuroscience, 2005, 8(3)365-371.

[14] Jasmin L, Rabkin S D, Granato A, et al. Analgesia and hyperalgesia from GABA-mediated modulation of the cerebral cortex[J]. Nature, 2003, 424(6946)316-320.

[15] Sunter D, Hewson A K, Lynam S, et al. Intracerebroventricular injection of neuropeptide FF, an opioid modulating neuropeptide, acutely reduces food intake and stimulates water intake in the rat[J]. Neuroscience letters, 2001, 313(3)145-148.

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